ExoTec MUSE Exosomes
Comprehensive specification documentation, characterization analytics, and formulation profile for Wharton Jelly Exosomes (ExoTec™) derived from healthy full-term human umbilical cords.
Laboratory
Samples.
Specification
- Particle size by NTA: 130±40 nm
- Purity: ≥ 97 %
- Identity: Complies
- Sterility: Sterile
- Endotoxin level: <10 EU/mg
- DNA/RNA concentration: > 400 ng/mL
- Protein concentration: > 800 µg/mL
- Exosomal markers: CD9 / CD63 / CD81
- Additional EV markers: TSG101 / ALIX
- Source-cell verification: MUSE / SSEA-3-positive [source verification required]
Lot Result
- Particle size by NTA: 125 nm
- Purity: ≥ 99,5 %
- Identity: Complies
- Sterility: Complies
- Endotoxin level: <2.3 EU/mg
- DNA/RNA concentration: >1876 ng/mL
- Protein concentration: > 1800 µg/mL
- Exosomal markers: Verified
- Additional EV markers: Verified
- Source-cell verification: MUSE / SSEA-3-positive
Formulation
0.22 µm-filtered solution containing:
- — 20 mM glycine,
- — 100 mM Trehalose,
- — 200 mM Mannitol,
- — 20 mM NaH2PO4 (pH 7.4);
As lyophilized powder.
Exosome count per vial:
100±10 billion per mL
Purity and identity was determined by Nanosight LM10 measurements. Protein was measured using Lowry standardized assay, DNA/RNA concentration was measured using standardized Qbit analysis assay. Sterility test of vialed product was performed according to Eur.Pharm. (Inoculation method). Endotoxin was determined using the gel clot assay according to Eur.Pharm.
Cargo & Protein Profile
ExoTec™ vesicles are highly characterized to ensure reliable expression patterns in research. Standard marker expression includes tetraspanins CD9, CD63, and CD81, which are verified via Western Blotting and flow cytometry. Additionally, internal markers TSG101 and ALIX confirm the endosomal biogenesis pathway typical of true exosomes.
- CD9 / CD63 / CD81 TetraspaninsPositive (>99% expression)
- TSG101 & ALIX Endosomal MarkersComplies / Confirmed
- Total Protein Concentration> 1800 µg/mL
- Nucleic Acid (DNA/RNA) Conc.> 1800 ng/mL
Quality & Reconstitution
To preserve structural integrity and prevent cargo leakage, ExoTec™ is supplied as a lyophilized powder. Reconstitution is recommended with our sterile buffer (20 mM glycine, 100 mM Trehalose, 200 mM Mannitol, 20 mM NaH2PO4 at pH 7.4). The vialed material exhibits high sterility and ultra-low endotoxin levels (<2.3 EU/mg), suitable for downstream cell-culture assays.
Research Use Warning
Supplied strictly for **Research Use Only (RUO)**. This product is not intended for injection, in vivo diagnostics, clinical testing, or human application. All handling must follow Biosafety Level 1 (BSL-1) lab regulations.